HTL 519 - Advanced Lab Methods in Histology and Pathology

3 Credit Hours
The techniques described and discussed are procedures in immunohistochemistry and in situ hybridization. Preparatory techniques for quantitative staining methods, enzyme histochemistry, and preparatory methods in cytopathology are stressed. The methods are taught in detail with conceptual principles of the technique and background material presented for each topic. Various methods for antigen detection are presented including avidin-biotin complex (ABC), peroxidase-antiperoxidase method and chromagen alternatives. Interpretation of results and problem-solving approaches are presented along with aids for troubleshooting. Antibody selection and quality control elements are discussed. Specialty enzymatic reaction stains, tissue preparation protocols, and direct smears for cytology are covered.